Large deletions induced by Cas9 cleavage
نویسندگان
چکیده
منابع مشابه
Large-scale genomic deletions mediated by CRISPR/Cas9 system
Owing to its simplicity of manipulation and high efficiency, the CRISPR/Cas9 technology has already been achieved as a robust genome engineering tool for generating gene mutations, multiple-gene knockout and knock-in in various species. However, tissues and genomic mosaicism have been widely reported in the gentically modified animals generated from zygotes that were coinjected with Cas9 mRNA a...
متن کاملLarge chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice
The Cas9/sgRNA of the CRISPR/Cas system has emerged as a robust technology for targeted gene editing in various organisms, including plants, where Cas9/sgRNA-mediated small deletions/insertions at single cleavage sites have been reported in transient and stable transformations, although genetic transmission of edits has been reported only in Arabidopsis and rice. Large chromosomal excision betw...
متن کاملIncreasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus
BACKGROUND The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is often limited by cell damage due to the endonuclease activity of the introduced Cas9 and the potential off-target DNA cleavage from incorrect guide by the 20 nt spacer. RESULT...
متن کاملLarge Genomic Fragment Deletions and Insertions in Mouse Using CRISPR/Cas9
ZFN, TALENs and CRISPR/Cas9 system have been used to generate point mutations and large fragment deletions and insertions in genomic modifications. CRISPR/Cas9 system is the most flexible and fast developing technology that has been extensively used to make mutations in all kinds of organisms. However, the most mutations reported up to date are small insertions and deletions. In this report, CR...
متن کاملCLN8 disease caused by large genomic deletions
BACKGROUND The presence of deletions can complicate genetic diagnosis of autosomal recessive disease. METHOD The DNA of patients was analyzed in a diagnostic setting. RESULTS We present three unrelated patients each carrying deletions that encompass the 37 kb CLN8 gene and discuss their phenotype. Two of the cases were hemizygous for a mutant allele - their deletions unmasked a mutation in ...
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ژورنال
عنوان ژورنال: Nature
سال: 2018
ISSN: 0028-0836,1476-4687
DOI: 10.1038/s41586-018-0380-z